Scientific Society of Agricultural SciencesJournal of Plant Production Sciences2314-79898120191101Diallel Cross Analysis for some White Maize Inbred Lines under Two Nitrogen Levels1105960210.21608/jpps.2019.59602ENM. M. Kamara S. A. OkashaKh. A. M. IbrahimJournal Article20190320A half diallel cross among seven white inbred lines of maize was made in 2016 growing season. The resulted of 28 F<sub>1</sub> crosses along with the check hybrid SC130 were evaluated under two different nitrogen levels, <em>i.e.,</em> 90 and 120 kg N/ fed using a randomized complete block design (RCBD) with three replications at the Experimental Farm, Faculty of Agriculture, Kafrelsheikh University in 2017 growing season, to estimate general (GCA) and specific (SCA) combining ability effects and their interactions with nitrogen levels as well as identify type of gene action controlling the inheritance of the studied traits. Data were recorded on days to 50% silking, plant height, ear height, ear length, ear diameter, No. of rows/ear, No. of kernels/rowand grain yield (ard/fed). The results showed that, the mean squares due to nitrogen levels (D), genotypes (G), crosses (Cr.), G × N and Cr. × N interactions were significant for all the studied traits. General (GCA) and specific (SCA) combining ability mean squares were highly significant for all the studied traits under both and across nitrogen levels. Both GCA and SCA effects were significantly interacted with nitrogen levels for all the studied traits, except GCA × N for ear diameter. The non-additive gene action played an important role in the inheritance of all the studied traits under the two nitrogen levels and the combined data, except days to 50% silking under N1 level. The inbred lines P<sub>1 </sub>and P<sub>7</sub> showed the best desirable GCA effects for earliness and P<sub>2 </sub>and P<sub>6</sub> for shorter plants and lower ear placement. Whereas, P<sub>2,</sub> P<sub>3 </sub>and P<sub>4</sub> were the best general combiners for grain yield. The crosses P<sub>1</sub>×P<sub>3</sub>, P<sub>2</sub>×P<sub>7</sub>, P<sub>3</sub>×P<sub>6</sub>, P<sub>4</sub>×P<sub>5</sub> and P<sub>5</sub>×P<sub>7</sub> had the best SCA effects for grain yield as well as one or more of its components under both and across nitrogen levels. The two crosses P<sub>2</sub>×P<sub>7</sub> and P<sub>4</sub>×P<sub>5</sub> exhibited significant and positive superiority over SC 130 under both and across nitrogen levels.https://jpps.journals.ekb.eg/article_59602_bde9b282ae8db797170089699f87cb7b.pdfScientific Society of Agricultural SciencesJournal of Plant Production Sciences2314-79898120191101Effect of Different Levels of Humic Acid and Mineral Fertilizers on Growth and Productivity of Sunflower11185960510.21608/jpps.2019.59605ENMourad, KH. A. DW. MA. K. TeilebJournal Article20190513Two field experiments were conducted out at Itay EL-Baroud Agricultural Research Station to investigate the effect of different levels of humic acid and mineral fertilization alone or in combination on two sunflower cultivars, Sakha 53 and G102 during two summer seasons 2016 and 2017. The treatments were, i.e. T1 (100% mineral fertilizers + zero humic acid), T2 (75% mineral fertilizers + 2 litters of humic acid/feddan), T3 (50% mineral fertilizers + 2 litter of humic acid/feddan), T4 (50% mineral fertilizers + 1 litter of humic acid/feddan) and T5 (0% mineral fertilizers + 2 litter of humic acid/feddan), foliar application of humic was done on 25, 35 and 45 day after sowing (DAS). The experiments were designed in RCBD using split plot with three replications. The results indicated that highly significant difference were found between sunflower cultivars, also found between fertilization treatments during the two seasons, Sakha 53 recorded the best values for plant height, head diameter , seed yield/plant and seed yield/fad, while Giza 102 achieved desirable values for days to flowering and oil %. The treatments T1 and T2 exhibited the best results for most studied characters such as plant height, head diameter (cm), stem diameter (cm), no. of seeds/plant, 1000-seeds weight/plant and seed yield/fad with exception of oil % and physiological maturity date. Best value of benefit cost ratio was obtained by T1 and T2 among treatments and Sakha 53 compared to Giza 102.https://jpps.journals.ekb.eg/article_59605_3533d0f3403ff6d238c1ed9b570bc178.pdfScientific Society of Agricultural SciencesJournal of Plant Production Sciences2314-79898120191101Evaluation of some Physiological and Molecular Characters of Salt-Tolerant Potato Mutants Induced by Gamma Irradiation19296784010.21608/jpps.2019.67840ENElsadany, O. M. M. A. Elhamahmy*; Manal Eid; Samah SabryS. M. GerishJournal Article20191210Four Potato cultivars (Lady Rosetta, Diamante, Gold and Santana) were treated with 20 Gray of gamma rays then screened for salt tolerance by application of 2270 ppm of NaCl <em>in vitro</em>. Evaluation of tolerant mutants was done by biochemical and molecular analysis. Results showed that, Ch.a and b were increased in gamma treated-plantlets compared to control and the highest values of Ch.a was found in Lady Rosetta, Diamante and Santana cvs, while ch.b as well as Chl. a+b were highest in Diamante and Santana cvs. Phenolics and Carotenoids concentrations were also increased in plantlets as a result of gamma application especially in Santana and Lady Rosetta cvs., respectively. Free amino acids concentration had variable effect between gamma treated and non-treated plantlets, so it increased in Santana and Gold cvs., it decreased in Lady Rosetta and Diamante cvs. Superoxide dismutase and Catalase activity were also increased in all gamma-treated cultivars especially in Lady Rosetta, Diamante and Gold cvs. SDS-PAGE of protein revealed that, gamma-treated and untreated plantlets expressed about 14 to 17 protein bands. Gamma-treated plantlets of Lady Rosetta cv. missing two protein bands with 55 and 6 KDa compared to control, Gamma-treated plantlets of Gold cv. lost three protein bands with 23, 17 and 6 KDa compared to untreated ones while the band with 55 KDa was detected in gamma treated-plantlets. We can conclude that, using of 20-gray gamma rays' dose was more effective tool for induction salt-tolerant mutant plantlets of potato cultivars especially with Santana cv. and such mutants must be re-evaluated on the field experiments.https://jpps.journals.ekb.eg/article_67840_f44fd551ca78c9433576a65a326b1294.pdfScientific Society of Agricultural SciencesJournal of Plant Production Sciences2314-79898120200110Characterization of ISSR and SCoT Markers and TaWRKY Gene Expression in some Egyptian Wheat Genotypes under Drought Stress314672549ENDiaaAbd El-MoneimDepartment of Plant Production (Genetic branch), Faculty of Agricultural and Environmental Sciences, Arish University, Arish, 45511, EgyptJournal Article20191227Ten ISSR and SCoT primers were used to estimate the genetic variability between some Egyptian wheat genotypes. A total of (141) bands across both types of markers, of which 72 ISSR bands (87.5%) and 69 SCoT bands (81.1%) were polymorphic. ISSR showed higher levels of polymorphism (P%), indicating its efficacy in separating closely related germplasm. The polymorphism information content (PIC) and resolving power (Rp) indicated no preference for any type of markers. Effective multiplex ratio (EMR), marker index (MI) indicated that ISSR revealed higher values. SCoT1 primer showed the highest P%, PIC, MI and EMR values while SCoT12 showed the highest Rp values. While, HB-11 primer showed the highest MI and EMR values, 98-A primer showed the highest P% and PIC. Across the two types of markers, a total of 54 genotype-specific markers were amplified. Most markers were showed by Shandaweel 1 genotype. Some of these markers are related to drought tolerance, and also, can be used in detecting possible relatedness among genotypes. We had profiled the expression of seven <em>TaWRKY</em> genes under PEG6000 stress. High variation in gene expression was observed between Shandaweel 1and Misr 3. All <em>TaWRKY </em>genes were expressed under different concentrations of PEG for Shandaweel 1, while Misr 3 was up regulated for all studied genes except for <em>TaWRKY50</em>. The relative <em>TaWRKY</em> genes expression showed highest and lowest levels in Shandaweel 1 and Misr 3 respectively. Moreover, <em>TaWRKY44</em> upregulated under all studied concentrations of PEG except for Shandaweel 1 at 15 % PEG, while <em>TaWRKY50</em> was downregulated for both genotypes under all studied concentrations except for Shandaweel 1 at 15 %PEG. Generally, we demonstrated high genetic variability through DNA marker, and variable gene expression studies between the studied genotypes.https://jpps.journals.ekb.eg/article_72549_8602645b0cf49b4460ecda56bf84cd26.pdf